The NanoOne 250 by UpNano is a high-resolution 3D printer based on two-photon polymerization (2PP) technology, enabling the fabrication of micro- and nanoscale structures with exceptional precision and speed.

Function:
Using femtosecond laser pulses and two-photon absorption, the NanoOne 250 polymerizes photosensitive resins at extremely fine resolutions, allowing for the creation of complex 3D structures down to sub-micron detail.

Available Resins:
- UpPhoto: general purpose and high performance resin
- UpBrix: refractive index matched resin for ultra-high resolution applications
- UpOpto: optically transparent resin for photonic and micro-optical applications
- OrmoComp: hybrid organic-inorganic resin, ideal for biocompatible and medical-grade components

Note: Users may bring their own resin, but its use must be approved by facility staff in advance to ensure compatibility and safety.

Objectives:
- 60x
- 20x
- 10x
(choice based on desired resolution and build volume)

Printing Modes:
- VAT
- Bottom-up
- Top-down

Maximum heights:
- VAT: 40 mm
- Top-down & bottom-up (limited by objective working distance):
   10x: up to 700 µm
   20x: up to 250 µm
   60x: up to 100 µm

Additional specifications:
- Accessible writing area: up to 120 × 100 mm²
- Horizontal & vertical resolution: ≤ 10 nm
- Horizontal feature size:
   60x: ≤ 200 nm
   20x: ≤ 420 nm
   10x: ≤ 730 nm
- Vertical feature size:
   60x: ≤ 0,5 µm
   20x: ≤ 2,9 µm
   10x: ≤ 9,2 µm
- Writing speed (max):
   60x: 100 mm/s
   20x: 300 mm/s
   10x: 600 mm/s